Significance of detecting miR-145 hypermethylation in non-small cell lung cancer
10.3969/j.issn.1006-5725.2016.16.021
- VernacularTitle:miR-145高甲基化在非小细胞肺癌组织中检测的意义
- Author:
Yongmei CHANG
;
Mingzhi WANG
;
Cong SUN
;
Wensen YAN
;
Qingfeng LIU
;
Xiaoyun JIANG
;
Jun WANG
- Publication Type:Journal Article
- Keywords:
MiR-145;
Non-small cell lung cancer;
Hypermethylation;
Differentiation stage
- From:
The Journal of Practical Medicine
2016;32(16):2671-2673
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the significance of detecting miR-145 hypermethylation in non-small cell lung cancer (NSCLC). Methods Seventy-five NSCLC tissues and adjacent tissues was collected from May 2012 to January 2015. The methylation status of miR-145 promoter was tested by bisulfite sequencing PCR (BSP) and the expression of miR-145 evaluated by ISH. The correlation between miR-145 methylation and clinical parameters , and the relationship between miR-145 methylation and expression were statistically analyzed. Results The hypermethy-lation ratio of miR-145 in NSCLC tissues was higher than that in the adjacent tissues. Hypermethylation of miR-145 was correlated with NSCLC differentiation stage, but not with gender and age. In NSCLC, miR-145 hypermethyla-tion was negatively related with its expression. Conclusion MiR-145 hypermethylation is closely related with dif-ferentiation stage in NSCLC tissues. MiR-145 hypermethylation may be a potential biomarker for estimating NSCLC differentiation stage.
