Effect of ERK1/2 Phosphorylation on Proliferation of Human Aortic Vascular Smooth Muscle Cells stimulated by Advanced Glycation End Products
10.3969/j.issn.1006-5725.2016.15.003
- VernacularTitle:晚期糖基化终末产物激活ERK1／2信号通路参与人主动脉血管平滑肌细胞增殖
- Author:
Gang YUAN
;
Yong LIU
;
Hong ZENG
;
Xiaolei SUN
;
Huqiang HE
;
Lei ZHANG
;
Qingchun HOU
;
Kaiqiang XU
- Publication Type:Journal Article
- Keywords:
Advanced Glycation End Products;
ERK1/2 Phosphorylation;
Proliferation;
Smooth Muscle Cell
- From:
The Journal of Practical Medicine
2016;32(15):2430-2433
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of ERK1/2 phosphorylation on the proliferation of human aorta vascular smooth muscle cells (HAVSMCs) stimulated by advanced glycation end products (AGEs) Methods CCK8 was used to test the effect of AGEs with different concentration on the proliferation of HAVSMCs, and the effect of PD98059, a specific inhibitor of ERK1/2, on HAVSMCs proliferation stimulated by AGEs was also detected. Flow Cytometer (FCM) was used to detect the cell cycle transformation induced by AGEs. Western Blot was used to detect the expression of relative proteins. Results 10 mg/L AGEs observably facilitated the proliferation and the DNA synthesis of HAVSMCs and PD98059 (40 umol/L) markedly inhibited the proliferation and cell cycle evolution of HAVSMCs induced by AGEs. Furthermore, ERK1/2 phosphorylation, and PCNA were regulated by AGEs and thus it showed time and dose dependent. Conclusion AGEs participates in the proliferation of HAVSMCs by activating ERK1/2 signal path.
