Effect of RU486 on pre-adipocytes differentiation and NF-κB activation
10.3969/j.issn.1006-5725.2016.15.004
- VernacularTitle:米非司酮对前脂肪细胞的分化及核因子κB激活的影响
- Author:
Yifei WANG
;
Na YI
;
Linying WU
;
Xiaochun HUANG
;
Sihong LIANG
- Publication Type:Journal Article
- Keywords:
RU486;
NF-κB;
Adipocytes differentiation;
3T3-L1 cells
- From:
The Journal of Practical Medicine
2016;32(15):2434-2437
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the roles of RU486 inhibiting 3T3-L1 pre-adipocytes differentiation and regulating NF-κB activation. Methods Cells were treated with RU486 with concentrations of 0.1 ~ 10μmol/L for 48 h , then the relative contents of triglyceride were analyzed by Oil-Red O staining assay on 9 th day during adipogenesis. The mRNA expressions of PPARγ2,C/EBPa, LPL and FAS were further measured by Real-time PCR. IκBα protein level was detected by Western bolt and nuclear translocation of NF-κB was observed by immunofluorescence assay. Results The relative contents of triglyceride decreased with the increasing of RU486 concentration. Compared with the control, the relative contents of triglyceride in RU486-treatment groups from 0.5 μmol/L were significantly decreased (P < 0.05 or P < 0.01). Compared with the control, PPARγ2, C/EBPa, LPL and FAS mRNA expression and IκBα protein level were significantly decreased (P < 0.01) and NF-κB nuclear translocated from cytoplasm to nucleus in Group 5 mmol/L RU486. Conclusions RU486 could down regulate IκBα protein level , activate NF-κB nuclear translocation , then down regulate PPARγ2 , C/EBPa , LPL and FAS mRNA expression and inhibit adipocytes differentiation.
