Application of Time-resolved Fluroimmunoassay for Determination of Furaltadone Metabolite 3-Amino-5-morpholinomethyl-2-oxazolidinone
10.11895/j.issn.0253-3820.160192
- VernacularTitle:呋喃它酮代谢物时间分辨荧光免疫分析法的建立与应用
- Author:
Lihua DENG
;
Jinbo DAI
;
Zhenlin XU
;
Jinyi YANG
;
Hong WANG
;
Zhili XIAO
;
Hongtao LEI
;
Yuanming SUN
;
Yudong SHEN
- Publication Type:Journal Article
- Keywords:
Furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone;
Time-resolved fluoroimmunoassay;
Europium Ⅲ-labeled antibody
- From:
Chinese Journal of Analytical Chemistry
2016;(8):1286-1290
- CountryChina
- Language:Chinese
-
Abstract:
To detect furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone ( AMOZ ) in fish sample, an Eu3+ labeling time-resolved fluoroimmunoassay ( TRFIA ) was developed. The effects of experimental conditions including AMOZA-OVA concentration, dilution of antibody, and reaction time on the sensitivity of TRFIA were explored. The results showed that the optimized assay conditions were as follows:the AMOZA-OVA concentration was 0. 25 μg/mL; the antibody was diluted 5í104 folds, and the competitive reaction time was 50 min. Under optimal conditions, the method showed a detection limit of 0. 01 ng/mL, an IC50 of 0. 26 ng/mL and a linear range (IC20-IC80) of 0. 025-2. 83 ng/mL. The recoveries of AMOZ in fish at three spiked levels ranged from 78 . 0% to 86 . 0%, and the relative standard deviations were less than 15%. Good correlation between the ic-TRFIA and high performance liquid chromatography-tandem mass spectrometry was obtained for spiked food samples. The proposed ic-TRFIA method was suited for the determination of AMOZ residue in food samples.
