Recent Development of Droplet Microfluidics in Digital Polymerase Chain Reaction
10.11895/j.issn.0253-3820.160074
- VernacularTitle:液滴微流控系统在数字聚合酶链式反应中的应用研究进展
- Author:
Yiqiang FAN
;
Mei WANG
;
Feng GAO
;
Jian ZHUANG
;
Gang TANG
;
Yajun ZHANG
- Publication Type:Journal Article
- Keywords:
Digital polymerase chain reaction;
Droplet microfluidics;
Genetic testing;
Gene sequencing;
Review
- From:
Chinese Journal of Analytical Chemistry
2016;(8):1300-1307
- CountryChina
- Language:Chinese
-
Abstract:
Digital polymerase chain reaction ( PCR) has been experiencing a rapid development during the past few years. Comparing with the traditional real-time quantitative PCR ( RT-qPCR) , using the same primer and probe, the accuracy for the absolute quantification of target gene is significantly improved. The development of digital PCR is directly related to the development of microfluidics. The integrated fluid circuit is an early combination of the microfluidics and digital PCR, which has a complicated fabrication process with high cost. Recently, researchers are trying to apply the droplet microfluidics in digital PCR, and the droplet microfluidic chip is able to generate millions of droplets within a short time. Each of these droplets containing no more than one target gene is a reaction chamber during the amplification process. After amplification, each droplet is tested to achieve the absolute quantification of the target gene. This paper reviews the recent progresses of droplet digital PCR, and the applications of droplet digital PCR in biological, medical and environmental fields.
