Bone marrow-derived mesenchymal stem cells improved prognosis in rats with extended liver resection
10.3760/cma.j.issn.1007-8118.2016.08.012
- VernacularTitle:骨髓间充质干细胞改善大鼠扩大肝切除预后的机制
- Author:
Weizheng REN
;
Maosheng SU
;
Xiaoxia DU
;
Li ZHAO
;
Yu CHENG
;
Xiaozhong WU
;
Wanqing GU
- Publication Type:Journal Article
- Keywords:
Bone marrow-derived mesenchymal stem cell;
Hepatectomy;
Ischemia/reperfusion injury;
Liver regeneration
- From:
Chinese Journal of Hepatobiliary Surgery
2016;22(8):546-551
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of mesenchymal stem cells (MSC) in an animal model combining ischemia-reperfusion with 85％ liver resection.Methods Eight-week-old male SD rats received BM-MSC by tail vein and then underwent 30-min ischemia followed by 85％ liver resection.The survival rate was monitored for 7 days after surgery.Liver regeneration was assessed on day 2 after hepatectomy.Liver damage,liver cell apoptosis,and cytokine expression in the first 24 h after hepatectomy were also assessed.Results BM-MSC mostly homed to the spleen.Transplantation significantly inhibited myeloperoxidase [(19.9 ± 6.0) mg/g vs.(41.4 ± 10.2) mg/g] and downregulated proinflammatory cytokines.BM-MSC significantly reduced the ALT and AST levels [AST (1 475 ± 275) IU/L vs.(2 550 ± 441) IU/L,P ＜ 0.05;ALT (738 ± 101) IU/L vs.(1 113 ± 268) IU/L,P ＜ 0.05].The attenuation of liver injury was also verified histologically 24 h after surgery.Liver cell apoptosis was markedly reduced.Moreover,BM-MSC infusion significantly promoted remnant liver regeneration.As a result,the survival rate was improved by BM-MSC treatment in this model (95％ vs 70％,P ＜ 0.05).Conclusion In an animal model combining ischemia-reperfusion with 85％ liver resection,BM-MSC infusion attenuated liver injury and promoted hepatocyte regeneration,resulting in improved survival rate.
