The expressions and the clinical significance of Treg and IL-35 in patients with cholangiocarcinoma
10.3760/cma.j.issn.1007-8118.2016.08.011
- VernacularTitle:胆管癌患者调节性T细胞及白介素-35的表达及临床意义
- Author:
Yizhen WANG
;
Haorun LIU
;
Li XIAO
;
Hongqiang ZHAO
;
Hang LIU
;
Hongtao CUI
;
Weimin LI
- Publication Type:Journal Article
- Keywords:
Cholangiocarcinoma;
Regulatory T cell;
Interleukin-35
- From:
Chinese Journal of Hepatobiliary Surgery
2016;22(8):542-545
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of regulatory T cell (Treg) and interleukin-35 (IL-35) in patients with cholangiocarcinoma and to explore their clinical significance.Methods Flow-cytometry,PCR,Enzyme-linked immunosorbent assay (Elisa) and immunohistochemistry were used to detect the levels of Treg and IL-35 in peripheral blood and cholangiocarcinoma tissues in 42 patients with cholangiocarcinoma.Healthy volunteers were used as a control group.Result The percentage of Treg cells to CD4 + T cells in patients with cholangiocarcinoma was (5.6 ± 1.7) ％,while that in the normal control group was (2.9 ± 0.8) ％.There was a significant difference between the two groups (P ＜ 0.05).The plasma levels of IL-35 in patients with cholangiocarcinoma was (198.4 ± 81.4) pg/ml,while that in the normal control group was (33.7 ± 18.0) pg/ml.Again,a significant difference was observed between the two groups (P ＜ 0.05).In peripheral blood mononuclear cell,the IL-35 mRNA level was positively correlated with the plasma IL-35 level (p35,R =0.795,P ＜0.05;EBI3,R =0.812,P ＜ 0.05).Immunohistochemical studies showed that FOXP3 + tumor cells and Treg cells increased significantly in tumor tissues.Conclusion Overexpressions of Treg and IL-35 in peripheral blood and tumor tissues of patients with cholangiocarcinoma suggested that they may play important roles in the development of cholangiocarcinoma.
