An application of DNA barcoding in identification of Cricetulus Barabensis
10.3760/cma.j.issn.2095-4255.2016.05.004
- VernacularTitle:DNA条形码技术在黑线仓鼠分类研究中的应用
- Author:
Baobao CHEN
;
Cuihong AN
;
Yangxin SUN
;
Suoping FAN
;
Lixia HUO
;
Wen LYU
;
Jianjun SHE
- Publication Type:Journal Article
- Keywords:
Cricetulus barabensis;
DNA barcoding;
Mitochondrial cytochrome C oxidase subunit I gene
- From:
Chinese Journal of Endemiology
2016;35(5):325-328
- CountryChina
- Language:Chinese
-
Abstract:
Objective To apply DNA barcoding technology for exploring its taxonomic status and differences in the molecular biology of Cricetulus barabensis in Shaanxi Province.Methods Sixty-five samples of Cricetulus barabensis were collected from Dingbian,Jingbian Counties in northern of Shaanxi and Dali County in Guanzhong plain (Dingbian 58 samples,Jingbian 2 samples,and Dali 5 samples).According to the mitochondrial cytochrome C oxidase subunit I gene (CO I) sequence,the genetic distance was calculated and Neighbor-Joining tree was constructed.Results The genetic distance between two samples (13.16,13.21) and other 56 samples of Dingbian was 9.2％-10.0％.The genetic distance between the 56 samples of Dingbian and Jingbian was less than 1％ and Dali was 7.2％-8.3％;the average intraspecific genetic distance of Jingbian and Dali was less than 1％.The Neighbor-Joining tree showed that all the Cricetulus barabensis samples from the three counties were separated into two large branches.The samples of 13.16,13.21 from Dingbian together were classified into a class and the rest of the samples into another separate branch.At the same time,other samples from Dingbian except 13.16,13.21 and Jingbian were distributed in a small branch,and Dali samples were occupied another small branch.Conclusion Using the DNA barcoding technology,we have determined three subspecies of Cricetulus barabensis in Shaanxi Province,Dingbian has two kinds and Dali has a different subspecies.
