Association between STAT3 gene and children with acute lymphoblastic leukemia
10.3760/cma.j.issn.2095-428X.2016.15.009
- VernacularTitle:STAT3基因与儿童急性淋巴细胞白血病的相关性
- Author:
Peiwei ZHAO
;
Xin YUE
;
Hao XIONG
;
Jianxin LI
;
Hui LI
;
Xuelian HE
- Publication Type:Journal Article
- Keywords:
Acute lymphoblastic leukemia;
STAT3 gene;
Polymorphism;
Child
- From:
Chinese Journal of Applied Clinical Pediatrics
2016;31(15):1152-1155
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association between single nucleotide polymorphism (SNPs) (rs1053005 and rs744166) and expression level of STAT3 gene and the susceptibility to acute lymphoblastic leukemia (ALL) in Chinese children.Methods A case-control study was performed,and 184 children with ALL and 377 healthy children as controls were recruited.The genotypes of 2 SNPs were detected by using polymerase chain reaction-restriction fragment length polymorphism method.And the expression level of STAT3 gene was detected by using real-time PCR;All the data were analyzed by using SPSS 16.0 software.Results (1) In this study,the genotypes (GG,AG,AA) of rs1053005 had a significant difference between the ALL group and control group (x2 =6.737,P =0.034).Compared with control group,the A allele had a higher frequency in ALL group and A allele was a risk factor(x2 =5.853,P =0.016).But,there was no difference in frequency of genotype rs744166 between the 2 groups (x2 =1.866,P =0.393).(2) There was no significant association between genotypes and risk degree among 3 groups (high risk group,medial risk group and standard risk group) (x2 =0.335,P =0.987).(3) The expression level of STAT3 gene in patients with AA genotype was lower than that of the patients with GG genotype (t =4.758,P =0.009);and compared with patients of the standard risk group,high risk patients had a lower expression level of STAT3 gene (t =5.284,P =0.007).Conclusions The polymorphism of SNP rs1053005 was associated with ALL,with A allele being a risk factor;and the expression level of STAT3 gene maybe associated with the risk degree in ALL patients.
