Study on radioprotection of indole-3-carbinol acid condensation products
10.3760/cma.j.issn.1673-4181.2016.03.004
- VernacularTitle:吲哚-3-甲醇酸性代谢产物辐射防护作用的研究
- Author:
Xiaofei CHU
;
Shuyi ZHAO
;
Ming CUI
;
Lu LU
;
Junling ZHANG
;
Qinghui MENG
;
Saijun FAN
- Publication Type:Journal Article
- Keywords:
Indole-3-carbinol acid condensation products;
Radiation injuries;
DNA damage and repair;
Apoptosis
- From:
International Journal of Biomedical Engineering
2016;39(3):144-148
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the radioprotective effect of indole-3-carbinol (I3C) acid condensation products.Methods Cell colony formation assay was used to determine cell survival rate,and Western Blot assay was employed to measure protein expression.Results Seven kinds of the I3C acid condensation products showed different radioprotective effect on normal fibrous epithelial cells 184A1,among which 24 h pre-treatment of CTET (1 μmol/L),LTET (1 μmol/L),HI-IM (1 μmol/L) and 3,3'-diindoly methane (DIM) (0.3 μmol/L) showed significant increase of cell survival rate following irradiation with γ-ray,and the difficence was statistically significant (P＜0.05,P＜0.01).However,CT (1 μmol/L),LTr-1 (1 μmol/L) and ICZ (1 μmol/L) showed no effect on cell survival rate caused by radiation (P＞0.05).Furthermore,CTET,LTET,HI-IM and DIM activated the phosphorylation of ATM,BRCA1 and NBS1 proteins.HI-IM significantly decreased radiation-caused cell death and apoptosis.Conclusions CTET,LTET,HI-IM,and DIM can significantly reduce the radiosensitivity in 184A1 cells,and the mechanism may be related to the regulation of DNA damage and the repair of protein phosphorylation.
