Study on fluorescence PCR-melting curve method for detecting CYP2C9 and VKORC1 gene polymorphism in Xinjiang Hui population
10.3969/j.issn.1673-4130.2016.17.005
- VernacularTitle:荧光PCR-熔解曲线法检测新疆回族人群CYP2C9和VKORC1基因多态性研究
- Author:
Shuhong CHAI
;
Li CHEN
;
Bao GU
;
Lihong TANG
;
Jing YUAN
;
Ruixue CHENG
;
Zhongman ZHANG
;
Yingfen ZHANG
- Publication Type:Journal Article
- Keywords:
Xinjiang Hui nationality;
gene polymorphism;
cytochrome P450 2C9;
vitamin K epoxide reductase complex subunit 1
- From:
International Journal of Laboratory Medicine
2016;37(17):2371-2373
- CountryChina
- Language:Chinese
-
Abstract:
Objective To use the fluorescence PCR‐melting curve method to detect CYP2C9 and VKORC1 gene polymorphism in Xinjiang Hui population ,to analyze their gene distribution and gene mutation frequency ,and to evaluate the clinical applicability of the fluorescence PCR‐melting curve method .Methods The fluorescence PCR‐melting curve method and sequencing method were adopted to contrastively detect CYP2C9*2 ,CYP2C9*3 and VKORC1(‐1639G/A)gene polymorphism .Results Among detected 228 Xinjiang Hui individuals ,199 cases of CYP2C9*1/*1 ,2 cases of CYP2C9*1/*2 ,26 cases of CYP2C9*1/*3 and only 1 case of CYP2C9*3/*3 were detected ,no case of CYP2C9*2/*2 and CYP2C9*2/*3 was detected .Two kinds of allele G and A were detected for VKORC1(‐1639G/A) ,in which VKORC1‐1639G/G type was detected in 2 cases ,VKORC1‐1639G/A type was detected in 39 cases and VKORC1‐1639A/A type was detected in187 cases ,compared with the sequencing method ,the results of the fluorescence PCR‐melting curve method were completely consistent .Conclusion Xinjiang Hui population also has CYP2C9 gene *2 ,*3 loci and VKORC1 gene(‐1639G/A) locus polymorphism ,their occurrence frequency has a certain difference with Xingjiang Uygur and other regional populations ,the adopted fluorescence PCR‐melting curve method used in the gene polymorphism detection can meet clinical detection requirements .
