Enhancement of T-type Ca2+ channel currents in dorsal root ganglia neurons by nesfatin-1 and possible mechanisms
10.3760/cma.j.issn.0254-9026.2016.05.021
- VernacularTitle:黑皮质素-1对背根神经节神经元T-型钙通道电流的影响及机制研究
- Author:
Jiaoqian YING
;
Yuan ZHANG
;
Guoqiang ZHANG
;
Jin TAO
;
Zhigang CHANG
- Publication Type:Journal Article
- Keywords:
Melanocortins;
Calcium channels,T-type;
Ganglia,spinal
- From:
Chinese Journal of Geriatrics
2016;35(5):543-547
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of nesfatin-1 (NSF-1) on T-type Ca2+ channel currents in adult mouse dorsal root ganglion (DRG) neurons and possible signal transduction mechanisms involved.Methods We measured the expression of melanocortin 4 receptors(MC4-R)in mouse DRG by using western blotting analysis.The whole-cell patch clamp technique was used to record the effects of NSF 1 on T-type Ca2+ channel currents in small DRG neurons and several ligands were experimented to further clarify relevant signaling pathways.Results MC4-Rs were abundantly expressed in DRG neurons.NSF-1 enhanced T-type calcium channel currents in a dose-dependent manner in small DRG neurons in mice.NSF-1 mediated increment of T-type calcium channel currents was blocked by the MC4-R antagonist HS024,phosphokinase C antagonists GF109203X,and chelerythrine chloride,while the blockade of phospohokinase A PKI 6-22 elicited no such effects.Conclusions NSF-1 can enhance T type calcium channel currents in small DRG neurons through an MC4-R-dependent PKC signaling pathway.