Effects of DNMT1-siRNA on the pain behavior and the expression of SOCS1,p-ERK,p-CERB in CCI rats
- VernacularTitle:鞘内注射DNMT1-siRNA对CCI大鼠行为学和脊髓SOCS1、p-ERK、p-CREB表达的影响
- Author:
Ting ZHANG
;
Kai SUN
;
Wei YIN
- Publication Type:Journal Article
- Keywords:
DNMT1;
SOCS1;
p-ERK;
p-CREB;
Neuropathic pain;
Spinal cord
- From:
The Journal of Clinical Anesthesiology
2016;32(9):919-922
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of DNMT1 on neuropathic pain behavior and neuropathic pain modulation.Methods Thirty-two male SD rats, weighing 200-250 g, were randomly assigned into 4 groups (n =8 each):sham operation group (group S),chronic constrictive injury group (group CCI),CCI+ DNMT1-siRNA group (group CDS),CCI+ control-siRNA group (group CCS).Group CDS were intrathcally injected of DNMT1-siRNA (2 μg/10 μl),and group CCS were intrathcally injected of control-siRNA 7,8,9 days after operation.Mechanical withdrawal threshold (MWT)and thermal withdrawal latency (TWL)were measured before operation and on day 3,5,7,9,12,14 after operation.The rats were then sacrificed and L4-L6 segments of the spinal cord were removed for determination of SOCS1,p-ERK,p-CREB expression using Western blot on day 14.Results Compared with group S,MWT and TWL in group CCI and CCS were significantly decreased on day 3,5,7,9,12,14 after operation (P <0.05).Compared with group CCS,MWT and TWL in group CDS were significantly increased on day 9,12,14 after operation (P < 0.05 ). Compared with group S and CDS,SOCS1 was significantly downregulated,p-ERK and p-CREB were significantly upregulated in group CCI and CCS (P <0.05 ).Conclusion Intrathcal injection of DN-MT1-siRNA significantly relieves neuropathic pain by upregulating SOCS1,downregulating p-ERK and p-CREB in rats spinal cords.
