Investigation on Methylation of Runx3 Gene in Patients with Chronic Atrophic Gastritis
10.3969/j.issn.1008-7125.2016.08.006
- VernacularTitle:慢性萎缩性胃炎患者 Runx3基因甲基化水平的研究
- Author:
Chunna ZHAO
;
Lili XIAO
;
Bei WANG
;
Yueguang WEI
- Publication Type:Journal Article
- Keywords:
Gastritis,Atrophic;
Runx3;
Methylation;
Biological Markers
- From:Chinese Journal of Gastroenterology
2016;21(8):470-473
- CountryChina
- Language:Chinese
-
Abstract:
Background:Chronic atrophic gastritis(CAG)is a kind of chronic gastritis with atrophic changes of gastric mucosa. The studies on peripheral blood biomarkers in CAG are rare. Aims:To investigate the methylation of peripheral blood CpG sites in Runx3 gene promoter region in CAG patients. Methods:Eighty-two mild CAG patients,73 moderate to severe CAG patients from June 2013 to May 2014 at Daqing Oilfield General Hospital were enrolled,and 45 patients with normal gastric mucosa were served as controls. The methylation of CpG sites in Runx3 gene promoter region was measured by MALDI-TOF-MS. mRNA expression of Runx3 was determined by fluorescent quantitative PCR,and the protein expression of Runx3 was determined by Western blotting. Results:Compared with the control group and mild CAG group,methylation levels of CpG13,CpG14 and CpG15 sites in Runx3 gene promoter region were significantly increased in moderate to severe CAG group(P < 0. 05),the mRNA and protein expressions of Runx3 were significantly decreased( P < 0. 05). No significant differences in methylation of CpG sites in Runx3 gene promoter region and mRNA and protein expressions of Runx3 were found between mild CAG group and control group( P >0. 05 ). Conclusions:The hypermethylation of peripheral blood CpG13,CpG14 and CpG15 sites in Runx3 gene promoter region can inhibit the expression of Runx3 in CAG patients,and can be used potentially as the biomarker for clinical staging of CAG.
