MicroRNA-21 Mediates the Promoting of Proliferation of Gastric Cancer Cells by Prostaglandin E2 through PTEN/Akt Pathway
10.3969/j.issn.1008-7125.2016.08.007
- VernacularTitle:MicroRNA-21通过PTEN/Akt通路介导前列腺素E2促进胃癌细胞的增殖
- Author:
Hao ZHOU
;
Shaoren ZHANG
;
Hongyan LIU
- Publication Type:Journal Article
- Keywords:
MicroRNAs;
Stomach Neoplasms;
Prostaglandins E;
PTEN/ Akt Pathway
- From:
Chinese Journal of Gastroenterology
2016;21(8):474-478
- CountryChina
- Language:Chinese
-
Abstract:
Background:Prostaglandin E2(PGE2 )could promote the proliferation of tumor cells,microRNA-21(miR-21)could inhibit the proliferation of tumor cells,but its signal pathway is still unclear. Aims:To investigate the mechanism of PGE2 on promoting proliferation of gastric cancer cells potentially mediated by miR-21. Methods:Gastric cancer AGS cells were cultured and divided into control group,PGE2 group,anti-miR-21 group and PGE2 + anti-miR-21 group. Cell proliferation was determined by WST-1 chromatometry. Cell apoptosis rate was detected by flow cytometry. The expression of miR-21 mRNA was detected by RT-PCR. After AGS cells were intervened by Akt specific inhibitor perifosine,cell proliferation was assessed,and expression of PTEN/ Akt protein was detected by Western blotting. Results:Compared with control group, survival rate of AGS cells was significantly increased(P < 0. 05),apoptosis rate was significantly decreased(P < 0. 05), and expression of miR-21 mRNA was significantly increased in PGE2 group(P <0. 05). Compared with PGE2 group, survival rate of AGS cells was significantly decreased(P < 0. 05),apoptosis rate was significantly increased(P < 0. 05), and expression of miR-21 mRNA was significantly decreased in anti-miR-21 group and PGE2 + anti-miR-21 group(P <0. 05). After intervention with perifosine,survival rate of AGS cells was significantly decreased(P < 0. 05),apoptosis rate was significantly increased(P < 0. 05),expression of PTEN protein was significantly increased,and expression of p-Akt protein was significantly decreased. Conclusions:MiR-21 mediates the promoting of proliferation of gastric cancer cells by PGE2 through PTEN/ Akt pathway,which might become a new target for the prevention and treatment of gastric cancer.
