Effect of amifostine on proliferation and differentiation of human megakaryocyte Dami cells
10.3867/j.issn.1000-3002.2016.07.003
- VernacularTitle:氨磷汀对人巨核细胞白血病Dami细胞增殖及分化的影响
- Author:
Haitao WANG
;
Bo YANG
;
Xuechun LU
;
Bo HU
;
Hongqi YANG
;
Longlong LUO
;
Jie LIN
;
Suxia LI
;
Hui FAN
;
Chunxia QIAO
;
Wei WANG
;
Xiaoling LANG
;
Jing GENG
;
Yan LI
;
Xiaoxiong WU
;
Ming LYU
;
Hongli ZHU
- Publication Type:Journal Article
- Keywords:
amifostine;
Dami cells;
cell differentiation;
CD41a;
DNA ploidy
- From:
Chinese Journal of Pharmacology and Toxicology
2016;30(7):723-727
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effect of amifostine(Amf)on the differentiation of human megakaryocyte cell line-Dami. METHODS Dami cells were treated with Amf 0.01-5.0 mmol · L-1 for 12 d. Dami cells were counted every day for the growth curve:only cells with a diameter>20μm. The platelet demarcation membrane system was observed by transmission electron microscopy. The expression of CD33,CD34,CD41a and DNA ploidy was detected by flow cytometry. RESULTS Amf 0.1-1.0 mmol · L-1 promoted the differentiation of Dami cells ,but inhibited their proliferation at a concentration>1.0 mmol · L-1. When these cells were treated with Amf 1.0 mmol · L-1 for 12 d,the platelet demarcation membrane system was observed,the percentage of cells with a diameter >20 μm was increased by 24.6%(P<0.01),the expression of CD41a was increased by 11.9%,while the expression of CD33 was decreased by 13.6%(P<0.05). Polyploidy cells(16N)were observed,and 4N,8N and 16N cells were increased to 31.56%,8.83% and 3.43%,respectively(P<0.05). CONCLUSION Amf 0.1-1.0 mmol · L-1 can promote the differentiation of Dami cells,but inhibit their proliferation at a high concentration(>1.0 mmol·L-1).
