Effects of Procyanidin on Myocardial Apoptosis and Related Protein Expressions in Experimental Rats With Myocardial Ischemia Reperfusion Injury
10.3969/j.issn.1000-3614.2016.07.018
- VernacularTitle:原花青素对缺血再灌注大鼠心肌细胞凋亡及凋亡相关基因蛋白表达的影响
- Author:
Dan LIU
;
Gaopin WANG
;
Qinghua CHANG
;
Yi LIU
- Publication Type:Journal Article
- Keywords:
Ischemia reperfusion;
Procyanidin;
Apoptosis
- From:
Chinese Circulation Journal
2016;31(7):696-700
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effects of procyanidins on myocardial apoptosis and related protein expressions of caspase-3, Bcl-2 and Bax in experimental rats with ischemia reperfusion (I/R) injury and to explore the possible mechanism. Methods: A total of 40 male SD rats were randomly assigned to 4 groups: Sham group, IR group, Low-dose procyanidin (50 mg?kg-1) group, and High-dose procyanidin (100 mg?kg-1) group. n=10 in each group and the rats were pre-treated by intra gastric drug administration once/day for 2 weeks, then left anterior descending artery (LAD) occlusion was conducted for 30 minutes followed by reperfusion for 120 minutes to establish IR model. Blood levels of CK-MB activity and myocardial infarction (MI) size were examined; protein expressions of caspase-3, Bcl-2 and Bax were determined by Western blot analysis; myocardial apoptotic index was measured by TUNEL method. Results: Compared with Sham group, IR group presented the higher CK-MB activity, enlarged MI size, increased index of apoptosis, elevated protein expressions of caspase-3 and Bax, while reduced protein expression of Bcl-2 and the ratio of Bcl-2/Bax, P<0.05. Compared with IR group, both Low-dose and High-dose procyanidin groups had the lower CK-MB activity, smaller MI size, decreased index of apoptosis, reduced protein expressions of caspase-3 and Bax, while elevated protein expression of Bcl-2 and the ratio of Bcl-2/Bax,P<0.05. Conclusion: Procyanidin could reduce myocardial apoptosis index in experimental IR rats, which might be related to decreased protein expressions of caspase-3 and Bax, increased protein expression of Bcl-2 and the ratio of Bcl-2/Bax.
