Evaluation of Chemiluminescence Immunoassay for Detecting Blood Levels of Aldosterone and Renin With its Diagnostic Value of Primary Aldosteronism
10.3969/j.issn.1000-3614.2016.06.020
- VernacularTitle:化学发光免疫法检测醛固酮和肾素浓度及对原发性醛固酮增多症诊断价值的评价
- Author:
Fang WANG
;
Jinsuo KANG
;
Yubao ZOU
;
Xiongjing JIANG
;
Xiangfeng CONG
;
Xi CHEN
- Publication Type:Journal Article
- Keywords:
Primary adlosteronism;
Chemiluminescence immunoassay;
Renin;
Aldosterone
- From:
Chinese Circulation Journal
2016;31(6):606-609
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate chemiluminescence immunoassay (CLIA) for detecting blood levels of aldosterone and rennin with its diagnostic value of primary aldosteronism (PA) with comparison to radio immunoassay (RIA). Methods: According to American protocols of CLSI, we conducted a veriifcation study between RIA and CLIA for their precision, accuracy, linearity and reference ranges; meanwhile, taking clinical diagnosis as golden standard, examined renin activity or concentration and aldosterone concentration in 20 healthy volunteers and 40 hypertension patients by both RIA and CLIA, compared the ratios of ARR (aldosterone concentration/renin activity) or ADRR (aldosterone concentration/renin concentration) for the speciifcity and sensitivity of PA diagnosis. Results: Within-lot and between-lot accuracies of CLIA for detecting aldosterone levels were below 5% and 10%, the recoveries were 102% and 95% respectively. There was a good linear correlation in the range of aldosterone at (3-74) ng/dl and renin at (0.99-330) μIU/ml. In healthy volunteers, renin level was higher in 2 subjects, while aldosterone level and ADRR ratio were within normal references in all subjects by the manufacturer. In hypertension patients, the sensitivity and speciifcity for aldosterone and rennin detections by CLIA were at 85.7% and 97.0%, by RIA were at 85.7% and 94.0%. Conclusion: CLIA has the superiority of simple performance, repeatable and without radioactive contamination; it is recommended for replacing RIA as necessity.
