Study on expressions of collagen Ⅰ and Ⅱ in aldosterone-induced human kidney tubular epithelial cells
10.3969/j.issn.1671-8348.2016.21.006
- VernacularTitle:醛固酮诱导人肾小管上皮细胞胶原Ⅰ、Ⅲ表达的研究
- Author:
Haiyang HUANG
;
Jiali WEI
- Publication Type:Journal Article
- Keywords:
aldosterone;
collagen-Ⅰ;
collagen-Ⅲ;
Rho kinase;
human proximal tubular epithelial cells;
tubular interstitial fi-brosis
- From:
Chongqing Medicine
2016;45(21):2900-2902
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of Rho kinase on collagen(COL)Ⅰ and Ⅲ expression of human renal tubular epithelial cells .Methods Human kidney tubular epithelial (HK‐2) cells were cultured in the RPMI‐1640 culture solution containing 15% fetal bovine serum .After 30 min pretreatment by the ALD receptor antagonist eplerenone(10 μmol/L) and Rho kinase inhibi‐tor Y27632(1 μmol/L) ,100 nmol/L ALD acted the HK‐2 cells for 24 h .The expressions of collagenⅠ and Ⅲ mRNA in each group were detected by real time PCR and the expression levels of COL Ⅰ ,Ⅲ and Rho kinase protein were detected by ELISA .Results ALD could up‐regulate the expressions of COLⅠ ,Ⅲ mRNA in HK‐2 cells ,and increased the levels of Rho kinase ,COLⅠ and Ⅲprotein ,while the Rho kinase inhibitor Y27632 and ALD receptor inhibitor eplerenone could antagonize these effects .Conclusion ALD could activate Rho kinase signal transduction pathway in HK‐2 cells and accelerate the progression of tubular interstitial fibro‐sis via Rho kinase induced expression of COL Ⅰ and Ⅲ .
