Altered expression of Chop and Grp78 in neural tube of rat with spina bifida
10.3760/cma.j.issn.1673-4408.2016.08.015
- VernacularTitle:Chop及Grp78基因在先天性脊柱裂大鼠胚胎12天神经管中的异常表达
- Author:
Di WU
;
Bo LIU
;
Yang FAN
- Publication Type:Journal Article
- Keywords:
spina bifida;
Chop;
Grp78;
Neuroepithelial stem cell
- From:
International Journal of Pediatrics
2016;43(8):651-653
- CountryChina
- Language:Chinese
-
Abstract:
Chop and Grp78 genes are key genes in endoplasmic reticulum stress(ERS).To explore the gene alteration of Chop and Grp78 in embryo with spina bifida,we studied Chop and Grp78 gene expression by qRT-PCR in neural tube of rat with spina bifida in E12 and primary cultured neuroepithelial stem cells(NEP).We found higher expression of both Chop and Grp78 in neural tube of rat with spina bifida than normal rats.While Chop and Grp78 genes were up-regulated after NEP differentiated into neuron.The up-regulation of Chop and Grp78 genes in neural rube of rat and NEP might be involved in the abnormal apoptosis in spina bifida related with ERS.And we found the primary culture of NEP provided a good model for embryo early development research.
