Expression and significance of miR-146a and LIN52 in advanced gastric cancer
10.3760/cma.j.issn.1006-9801.2016.06.007
- VernacularTitle:晚期胃癌患者miR-146a及LIN52的表达及其意义
- Author:
Yongxia CUI
;
Xiqing LI
;
Zhifen LUO
;
Chuangxin LU
;
Yanyan ZHU
;
Yun ZHOU
- Publication Type:Journal Article
- Keywords:
Gastric neoplasms;
miR-146a;
LIN52;
Treatment outcome;
Diagnosis;
Lymphatic metastasis
- From:
Cancer Research and Clinic
2016;28(6):388-393
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of miR-146a and its target gene LIN52 in advanced gastric cancer and their potential impact on clinical prognosis.Methods Total RNAs were extracted from 93gastric cancer tissues and their corresponding adjacent non-tumor tissues to quantify the relative expression of miR-146a by using real-time quantitative PCR (RT-qPCR).Expression of LIN52 was detected in tumors and normal tissues by immunohistochemistry.Correlation analysis was assessed between the expression of miR-146a and LIN52 and clinicopathological parameters,including clinical diagnostic specificity,tumor TNM staging,lymph node metastasis,differentiation grade,curative effect and prognosis of gastric cancer.Results The expression of miR-146a in gastric carcinoma was negatively correlated with lymph node metastasis (P ＜0.05).The expression of miR-146a had a significant correlation with the prognosis of the patients (P ＜ 0.01).The patients with high expression of miR-146a had higher survival rate (P ＜ 0.05),but the patients with high expression of LIN52 had lower survival rate (P ＜ 0.05).The receiver operating characteristic curve regression analysis showed that sensitivity and specificity of miR-146a were 94.1％ and 61.5 ％ to diagnose gastric cancer.Conclusions As a tumor suppressor gene in gastric cancer,miR-146a has significantly negative correlation with LIN52.High expression of miR-146a in the gastric cancer tissue might be associated with improved treatment efficacy of chemotherapy,suggesting that miR-146a may be a molecular marker for the diagnosis and prognosis of gastric cancer.
