Rosiglitazone-pretreated influenced the expression of peroxisome proliferator-activated receptor-γ,nicotinamide adenine dinucleotide phosphate: quinone oxidoreductase 1 and-γ-glutamylcysteine synthetase in microglia cells activated by thrombin in rats
10.3760/cma.j.issn.1006-7876.2016.07.006
- VernacularTitle:罗格列酮预处理对凝血酶激活的大鼠小胶质细胞过氧化酶活化增生受体γ、依赖还原型辅酶/Ⅱ醌氧化还原酶1及γ-谷氨酰半胱氨酸合成酶表达的影响
- Author:
Hang HANG
;
Likun WANG
;
Guofeng WU
;
Xingyu CHEN
- Publication Type:Journal Article
- Keywords:
Peroxisome proliferators;
Cerebral hemorrhage;
Rats;
Cells,cultured
- From:
Chinese Journal of Neurology
2016;49(7):536-542
- CountryChina
- Language:Chinese
-
Abstract:
Objective To activate the microglia cells by using thrombin,and then to observe the effect of precondition of rosiglitazone (RGZ)-pretreated on the expression change of peroxisome proliferator-activated receptor-γ (PPARγ),nicotinamide adenine dinucleotide phosphate:quinone oxidoreductase (NQO1) and γ-glutamylcysteine synthetase (γ-GCS).Methods Microglia cells were obtained from the brain tissues of the newborn rats and were primary cultured in vitro.The microglia cells were isolated in 14 days.The isolated microglia cells were randomly devided into normal control group (control group),thrombin stimulation group (stimulation group) and rosiglitazone intervention group (RGZ + TH group).The PPARγ,NQO1 and γ-GCS were observed by immunocytochemistry and real-time polymerase chain reaction (RT-PCR) methods.Results The immunocytochemistry showed that the number of stained cells of PPARγ,NQO1 and γ-GCS in stimulation group and RGZ + TH group were increased remarkably as compared with the control group.A significant increase of the PPARγ,NQO1 and γ-GCS was observed in the RGZ + TH group compared to the others.The RT-PCR method demonstrated that the expressions of PPARγ mRNA(211.88 ± 58.75),NQO1 mRNA(182.67 ± 62.09) and γ-GCS mRNA (188.17 ± 57.06) in RGZ + TH group were increased significantly as compared with the stimulation group (119.19 ± 44.58,101.73±32.19,108.81 ±19.71) or the control group (0.34±0.21,0.73±0.46,0.30±0.13;F=181.50,286.63,614.43,all P ＜ 0.01).Conclusion Medium-dose rosiglitazone-pretreated might increase the expression of PPARγ,NQO1 and γ-GCS in microglia cells activated by thrombin.Rosiglitazone might activate the PPARγso that increase its downstream gene to achieve its anti-oxidative stress effects.
