Effect of glucagon like peptide-1 on HSP70 expression of rabbit thoracic aortic endothelial cells under the influence of high glucose level
10.3760/cma.j.issn.1007-631X.2016.08.006
- VernacularTitle:胰高血糖素样肽-1对高糖环境下兔胸主动脉内皮细胞HSP70表达的影响
- Author:
Fanggang CAI
;
Pingfan GUO
;
Jie WU
;
Tenghui ZHAN
- Publication Type:Journal Article
- Keywords:
Glucagon-like peptide 1;
HSP70 Heat-shock proteins;
Aorta,thoracic;
Endothelial cells;
Diabetes mellitus
- From:
Chinese Journal of General Surgery
2016;31(8):632-635
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of GLP-1 on HSP70 expression of rabbit thoracic aortic endothelial cells at high glucose level.Methods Rabbit thoracic aortic endothelial cells were isolated and cultured.Tunnel was used to assay endothelial cells apoptosis.Cell proliferation was analyzed with BUDR.The protein level of HSP70 were detected by Western blot.Results The proliferation of rabbit thoracic aortic endothelial cells cultured under high glucose (33 mmol/L) decreased,(0.54 ±0.06 vs 0.78 ±0.04,P ＜ 0.01).The apoptosis and the protein level of HSP70 increased,(36.43％ ± 6.85％ vs 5.25％ ±0.73％) and (0.94 ± 0.11 vs 0.29 ± 0.03,all P ＜ 0.01).The proliferation of rabbit thoracic aortic endothelial cells (0.62 ± 0.06)in the GLP-1 group increased,the apoptosis ratio (10.13％ ± 1.19％) and HSP70 expression (0.76 ± 0.05) decreased significantly (P ＜ 0.05) compared with high glucose group.Conclusion GLP-1 can ameliorate high-glucose-induced rabbit thoracic aortic endothelial cells apoptosis and promote cell proliferation,attenuating high-glucose-induced level of HSP70 expression through the GLP-1 R-dependent pathways.
