Effect of recombinant human annexin A5 on expression of p-PKCα and p120-catenin during endotoxin-induced damage to cardiomyocytes
10.3760/cma.j.issn.0254-1416.2016.05.027
- VernacularTitle:重组人膜联蛋白A5对内毒素诱发心肌细胞员伤时p-PKCα和p120-catenin表达的影响
- Author:
Tao ZHAO
;
Changping GU
;
Mengjie LIU
;
Dong WANG
;
Fei ZHOU
;
Yanzhu GONG
;
Yuelan WANG
- Publication Type:Journal Article
- Keywords:
Annexin A5;
Endotoxins;
Myocytes,cardiac;
Protein kinase C-alpha;
Connexins
- From:
Chinese Journal of Anesthesiology
2016;36(5):610-612
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of recombinant human annexin A5 on the expression of phosphorylated protein kinase C alpha (p-PKCα) and p120-catenin during endotoxin-induced damage to cardiomyocytes.Methods H9c2 cells cultured in vitro were randomly divided into 3 groups (n=18 each) using a random number table:control group (group C),endotoxin group (group L),and recombinant human annexin A5 group (group A).Recombinant human annexin A5 (final concentration 5 ng/ml) was added,and the cells were incubated for 2 h in group A,and then lipopolysaccharide (final concentration 1 μg/ml) was added,and the cells were incubated for 4 h in L and A groups.At 4 h of incubation,cell apoptosis was detected using the cell apoptosis detection kit,the intercellular space was measured using the confocal microscopy,and the expression of p-PKCα and p120-catenin was determined by Western blot.The apoptosis rate was calculated.Results Compared with group C,the apoptosis rate was significantly increased,the intercellular space was significantly widened,the expression of p120-catenin was significantly down-regulated,and the expression of p-PKCα was significantly up-regulated in group L (P＜0.05).Compared with group L,the apoptosis rate and intercellular space were significantly decreased,the expression of p120-catenin was significantly up-regulated,and the expression of p-PKCα was significantly down-regulated in group A (P＜0.05).Conclusion Recombinant human annexin A5 can inhibit phosphorylation of PKCα and up-regulate the expression of p120-catenin,thus attenuating endotoxin-induced damage to cardiomyocytes.
