Effect of isoflurane anesthesia on Wnt3a expression in hippocampus of developing rats

VernacularTitle:异氟醚麻醉对发育期大鼠海马Wnt3a表达的影响
Author: Lining SUN ; Yue SUN ; Jun QI ; Xueqin WANG ; Kezhong LI
Publication Type:Journal Article
Keywords: Isoflurane; Infant,newborn; Wnt proteins; Hippocampus
From: Chinese Journal of Anesthesiology 2016;36(5):551-553
CountryChina
Language:Chinese
Abstract: Objective To evaluate the effect of isoflurane anesthesia on Wnt3a expression in the hippocampus of developing rats.Methods Twenty-four 7-day-old male Sprague-Dawley rats,weighing 125-155 g,were randomly divided into 3 groups (n=8 each) using a random number table:control group (group C),4 h inhalation of 2％ isoflurane group (group I4),and 6 h inhalation of 2％ isoflurane group (group I6).The rats were sacrificed at 6 h after the end of treatment in each group,and the hippocampi were removed for determination of Wnt3a mRNA expression (by real-time reverse transcriptase polymerase chain reaction) and Wnt3a expression (by Western blot).Results Compared with group C,the expression of Wnt3a and Wnt3a mRNA in hippocampi was significantly up-regulated in I4 and I6 groups (P＜0.05).Compared with group I4,the expression of Wnt3a in hippocampi was significantly up-regulated (P＜0.05),and no significant change was found in the expression of Wnt3a mRNA in hippocampi in group I6 (P＞0.05).Conclusion The mechanism of isoflurane-induced neurotoxicity is probably related to upregulation of Wnt3a expression in the hippocampal tissues of developing rats.