Effect of propofol on autophagy during oxygen-glucose deprivation/restoration in human liver cells
10.3760/cma.j.issn.0254-1416.2016.06.020
- VernacularTitle:异丙酚对人肝细胞氧糖剥夺-复氧复糖时自噬的影响
- Author:
Xiuya MA
;
Yujun SHEN
;
Yuanhai LI
;
Yuxian SHEN
- Publication Type:Journal Article
- Keywords:
Propofol;
Reperfusion injury;
Liver;
Autophagy
- From:
Chinese Journal of Anesthesiology
2016;36(6):725-727
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of propofol on autophagy during oxygen-glucose deprivation and restoration (OGD/R) in human liver cells.Methods Human hepatic HL-7702 cells at the logarithmic growth phase were seeded into culture plates and randomly divided into 3 groups (n =12 each) using a random number table:control group (group C),OGD/R group,and propofol + OGD/R group (group P+OGD/R).The cells were cultured in normal culture medium in group C.In OGD/R and P+OGD/R groups,the cells were subjected to O2-glucose deprivation for 6 h followed by restoration of O2-glucose supply for 12 h.Propofol with a final concentration of 50 mmol/L was added at 10 min before oxygen-glucose deprivation.The cell viability was detected by MTT assay.The expression of autophagy-related proteins such as microtubule-associated protein light chain 3 (LC3) and Beclin-1 was evaluated by Western blot.Immunofluorescence was used to determine the number and distribution of autophagosomes.Results Compared with group C,the cell viability was significantly decreased,the expression of LC3 and Beclin-1 was significantly up-regulated (P＜0.05),and the number of autophagosomes was significantly increased in OGD/R and P+OGD/R groups.Compared with group OGD/R,the cell viability was significantly increased,the expression of LC3 and Beclin-1 was significantly down-regulated (P＜0.05),and the number of autophagosomes was significantly decreased in group P+OGD/R.Conclusion The mechanism by which propofol reduces OGD/R injury is probably related to inhibition of autophagy in human liver cells.
