Radiation induced expression of HMGB1 in the cells with different radiosensitivity
10.3760/cma.j.issn.0254-5098.2016.07.002
- VernacularTitle:电离辐射对不同放射敏感性细胞中高迁移率簇蛋白B1的诱导表达作用
- Author:
Hong KANG
;
Hui YANG
;
Shaobo KE
;
Fuxiang ZHOU
;
Yunfeng ZHOU
- Publication Type:Journal Article
- Keywords:
High mobility group box 1;
Radioresistance;
Radiosensitive;
Ionizing radiation
- From:
Chinese Journal of Radiological Medicine and Protection
2016;36(7):486-490
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect ot ionizing radiation (IR) on the expressions of HMGB1 in the radiation-sensitive and radiation-resistant human cervical cancer cells and to analysis the role of HMGB1 in the regulation of radiosensitivity.Methods Human cervical cancer cells HeLa and its radioresistant strain HeLaR cells were irradiated with different doses of X-rays.The cells were collected at different time points after irradiation.The expressions of protein and mRNA of HMGB1 were detected by Western blot and real-time quantitative PCR.Results At the protein level,the expression of HMGB1 in HeLaR cells was significantly reduced at 6-36 h after 2,5 and 10 Gy X-ray irradiation (t =3.574-9.754,P ＜0.05),and then it was recovered to the control level at 48 h after IR.On the contrary,the expression of HMGB1 in HeLa cells was significantly increased at 6,12,48 h after 2 Gy IR (t =3.945-4.864,P＜0.05),at 6,36,48hafter5 GyIR (t=-2.875-3.295,P＜0.05),and at 36,48 h after 10 Gy IR (t =-4.480,-4.517,P ＜ 0.05).At mRNA level,the trend of HMGB1 expression alteration was consistent with that of protein expression.Conclusions The changes of HMGB1 expression can be differently induced by X-rays in the human cervical cancer radiation-sensitivity cells and radiation-resistant cells.HMGB1 may be involved in the radioresistance of human cervical cancer.
