Clinical research of detection of thrombosis in patients with lung cancer by protein fingerprinting
10.3760/cma.j.issn.1008-6315.2016.05.015
- VernacularTitle:蛋白质指纹技术检测肺癌患者血栓形成的临床研究
- Author:
Yuhui MA
;
Yi PEI
;
Lina JI
;
Yang DU
- Publication Type:Journal Article
- Keywords:
Deep venous thrombosis;
Lung cancer;
Protein fingerprint
- From:
Clinical Medicine of China
2016;32(5):437-439
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of serum protein fingerprint in patients with lung cancer with deep venous thrombosis.Methods Eighteen case patients with lung cancer were selected,including 8 case of lung cancers with thrombosis and 10 cases of lung cancers with no thrombosis.Surface enhanced laser desorption ionization protein-time of flight mass spectrometry (SELDI-TOF-MS) was used to analyze serum protein content of two groups in the same mass to charge ratios(M/Z),then drew the protein peaks that content difference was statistically significant.Results The M/Z of lung cancer with thrombus group and control group were 5911,1216,4187,1019,4293,the protein peaks had significant differences between the two group (43.81±7.74,6.37±5.02,2.97±0.35,35.96± 12.10,9.65±4.37;15.35± 12.69,2.06±0.37,4.67± 1.35,15.94±6.47,14.65±8.80;t =5.334,4.800,2.981,4.639,4.596;P＜ 0.05).Compared with the control group,the decrease of protein peak M/Z were 5911,1019,1216,and the increase of protein peak M/Z were 4293,4187 in the deep venous thrombosis group.Conclusion In the serum of patients with tumors SELDI profiles M/Z are 5911,4293,4187,1019,1216 of SELDI protein fingerprinting can be considered in patients with thrombosis of tumor specific markers.
