Effect of hsa-miR-663 on proliferation and apoptosis of human adrenal cortical cell line SW-13
10.3760/cma.j.issn.1000-6702.2016.08.016
- VernacularTitle:微小核糖核酸hsa-miR-663对肾上腺皮质细胞SW-13增殖及凋亡的影响
- Author:
Xin ZHAO
;
Xuebin ZHANG
;
Hanzhong LI
- Publication Type:Journal Article
- Keywords:
Cushing syndrome;
Adrenocorticotropin-independent macronodular adrenal hyperplasia(AIMAH);
Cell proliferation;
Cell apoptosis;
MicroRNA hsa-miR-663
- From:
Chinese Journal of Urology
2016;37(8):621-624
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of has-miR-663 on proliferation and apoptosis of human adrenal cortical cell line SW-13.Methods Based on the first stage study,in adrenocorticotropinindependent macronodular adrenal hyperplasia (AIMAH),hsa-miR-663 was up-regulated.The synthetic hsa-miR-663 mimics and inhibitors were transfected the SW-13 cell line.Real-time quantitative PCR (RT-PCR) tested the effect of the transfection.According to the results of RT-PCR,cell line was divided into five groups,including blank group,mimics NC group,inhibitor NC group,mimics group,inhibitor group.Then proliferating of the cell was tested by MTT and the apoptosis by Annexin V-FITC/PI.Result By RT-PCR,mimics group showed that has-miR-663 was over-expressed,and inhibitor group were knocked down.MTTshowed that,compared with NC group and Blank group,SW-13 cell line proliferated faster in mimics group and slower in inhibitor group.Annexin V-FITC/PI showed that apoptosis of SW-13 cell line was not different in all the five groups.Conclusion Up-regulating has-miR-663 could accelerate proliferation of adrenal cortical cells.The microRNA might play some role in the pathogenesis of AIMAH.
