A comparative study of DNA barcode of plague host animals between different foci
10.3760/cma.j.issn.2095-4255.2016.07.007
- VernacularTitle:不同疫源地鼠疫宿主动物的DNA条形码比较研究
- Author:
Fang LIU
;
Zili CHANG
;
Yanhong HU
;
Zhenghua WU
;
Jianyun LI
;
Zhidong MA
;
Zhongbing ZHANG
- Publication Type:Journal Article
- Keywords:
Genetic distance;
DNA barcode;
Plague
- From:
Chinese Journal of Endemiology
2016;35(7):498-501
- CountryChina
- Language:Chinese
-
Abstract:
Objective To illustrate more accurately the feasibility of DNA barcode technology in species identification in different geographical areas.Methods Four plague host animals were selected from three plague natural foci in Inner Mongolia for DNA barcode comparison,GC base content,codon preference and genetic distance calculation,and a phylogenetic tree was constructed.Results Compared between different foci of A llactaga sibirica,the difference of base content was statistically significant (F =5.34,P ＜ 0.05);the variation of Meriones unguiculatus focus Allactaga sibirica base content was larger,and compared with other foci Allactaga sibirica,the difference was statistically significant (all P ＜ 0.05).Different foci between one species rarely exist in codon sites.The genetic distance of intraspecific was less than 1％,and the genetic distance of interspecific was more than 18％.Conclusions DNA barcode of each species is specific,with no interspecific species sharing,and does not change with geographical position.Molecular identification of plague host animal is effective by DNA barcode.
