Studies on the long non-coding RNA during the reprogramming of human pluripotent stem cells

VernacularTitle:人诱导多能干细胞重编程过程中长链非编码RNA的差异表达研究
Author: Qian YU ; Yali LIU ; Yong FAN
Publication Type:Journal Article
Keywords: multipotent stem cells; nuclear reprogramming; microchip analytical procedures; long non-coding RNA
From: Tianjin Medical Journal 2016;44(7):843-845
CountryChina
Language:Chinese
Abstract: Objective To investigate the changes and roles of the long non-coding RNA (IncRNA)during the reprogramming of human induced pluripotent stem cells. Methods Agilent Human lncRNA (4 × 180K) chip was used to check the expression of lncRNA in somatic cells, induced pluripotent stem cells and embryonic stem cells. Compared with differentially expressed lncRNA in somatic cells and induced pluripotent stem cells, lncRNA was selected that may play an important role during the reprogramming of human pluripotent stem cells. Results The lncRNA expression profiles in induced pluripotent stem cells were similar to embryonic stem cells, but were different from the somatic cells. A total of 3 156 differentially expressed lncRNAs were found between stem cells and somatic cells by cluster analysis, and 222 differentially expressed lncRNAs were found during the reprogramming process of human pluripotent stem cells by biological analysis. Conclusion lncRNA may play an important role in reprogramming process of human pluripotent stem.