Extracelluar pH values influence high-phosphorus-induced VSMCs calcification mediated by BMP-2 signaling pathway
10.11958/20150117
- VernacularTitle:胞外pH值通过BMP-2信号通路影响高磷诱导的血管平滑肌细胞钙化
- Author:
Huiran ZHANG
;
Jinsheng XU
;
Liping GUO
;
Yaling BAI
;
Shenglei ZHANG
;
Junxia ZHANG
;
Liwen CUI
- Publication Type:Journal Article
- Keywords:
myocytes,smooth muscle;
hydrogen-ion concentration;
bone morphogenetic proteins;
vascular calcification;
pH;
BMP-2 signaling pathway
- From:
Tianjin Medical Journal
2016;44(7):861-864
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of different pH values on calcification of rat vascular smooth muscle cells (VSMCs) through bone morphogenetic protein (BMP)-2 signaling pathway. Methods Healthy male SD rats aged 5-8 weeks were selected in the study. VSMCs from rat thoracic aorta were cultured in vitro, and then identified by immunocytochemistry. The VSMCs were randomly divided into 4 groups by random sampling method:normal group (pH 7.4), pH7.4+high phosphorus group, pH 7.1+high phosphorus group, and pH 7.7+high phosphorus group. Calcium deposition and alkaline phosphatase (AKP) activity were measured by alizarin red staining and enzyme linked immunosorbent assay. The expressions of BMP-2, Smad1 and Runx2 mRNA were detected by RT-PCR. Results Compared with the control group, the calcification staining was increased in pH 7.4+high phosphorus group, calcium content was increased and expressions of BMP-2, Smad1, Runx2 mRNA and AKP activity were also increased (P<0.05). While compared with the pH 7.4+high phosphorus group, calcification staining, calcium content, expressions of BMP-2, Smad1, Runx2 mRNA and AKP activity were decreased in pH 7.1+high phosphorus group (P<0.05). The calcification staining, calcium content, expressions of BMP-2, Smad1, Runx2 mRNA and AKP activity were increased in pH 7.7+high phosphorus group (P<0.05). Conclusion The extracellular acidic environment (pH 7.1) can inhibit high-phosphotus-induced VSMCs calcification, whereas extracellular alkaline environment (pH 7.7) induce high-phosphotus-induced VSMCs calcification. The mechanism is presumably that VSMCs calcification is induced by influencing BMP-2 pathway, which may be mediated by VSMCs phenotype transdifferentiation of BMP-2 signaling pathway.
