Expression and significance of miR-193b in cervical cancer
10.3969/j.issn.1000-4718.2016.07.014
- VernacularTitle:miR－193b在宫颈癌中的表达与功能及其对化疗敏感性的影响
- Author:
Wenxia DU
;
Xia JI
- Publication Type:Journal Article
- Keywords:
MicroRNA-193b;
Cervical cancer;
HeLa cells;
Diamminedichloroplatinum
- From:
Chinese Journal of Pathophysiology
2016;32(7):1241-1245
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the expression of microRNA-193b(miR-193b) in the cervical tissues, and fur-ther to explore the effect of silencing miR-193b on diamminedichloroplatinum ( DDP)-treated HeLa cell viability.METH-ODS:The expression levels of miR-193b in different cervical tissues were examined by qPCR.After transfection of miR-193b-inhibitor, the cell migration was determined by Transwell assay, the sensitivity of HeLa cells to DDP was measured by MTT assay, the protein levels of phosphate and tension homology deleted on chromsome ten ( PTEN), protein kinase B (Akt), p-Akt and p-glycoprotein(P-gp) were determined by Western blot.RESULTS:The mRNA level of miR-193b was significantly increased in the cervical cancer tissues compared with normal cervical tissues ( P<0.05) .Knockdown of miR-193b obviously inhibited migration and enhanced sensitivity to DDP of HeLa cells (P<0.05).Additionally, after transfec-tion of miR-193b-inhibitor, the expression of PTEN was increased, whereas the protein levels of p-Akt and P-gp were de-creased (P<0.05).CONCLUSION:miR-193b is highly expressed in the cervical cancer tissues.Inhibition of miR-193b augments the sensitivity to DDP of HeLa cells, at least in part, through PTEN-PI3K/Akt signaling pathway.
