Combined effect of octreotide and Dachaihu decoction on treatment of se-vere acute pancreatitis
10.3969/j.issn.1000-4718.2016.07.023
- VernacularTitle:生长抑素联合大柴胡汤对重症急性胰腺炎大鼠的作用
- Author:
Hui FENG
;
Biao WU
;
Xide ZHAO
;
Baorui GUO
- Publication Type:Journal Article
- Keywords:
Severe acute pancreatitis;
Octreotide;
Dachaihu decoction;
NF-κB;
ICAM-1;
IL-1
- From:
Chinese Journal of Pathophysiology
2016;32(7):1297-1301
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To investigate the combined effect of octreotide and Dachaihu decoction on the treatment of severe acute pancreatitis (SAP).METHODS:Wistar rats (n=50) were randomly divided into sham group, SAP group, octreotide group, Dachaihu decoction group and combination group.The quantity of ascites was measured.The levels of amylase, alanine aminotransferase and creatinine in the serum were examined.The morphological changes of the pancreatic tissues were observed by HE staining.The activation of NF-κB and IκBαexpression were determined by Western blot.The mRNA expression with ICAM-1 and IL-1 was detected by qPCR.RESULTS: Combined treatment with octreotide and Dachaihu decoction effectively reduced the quantity of ascites and the levels of amylase, alanine aminotransferase and creat-inine in the serum in SAP rats.Moreover, combined treatment significantly inhibited SAP-induced activation of NF-κB and decrease in IκBαprotein expression, accompanied by a decrease in ICAM-1 and IL-1 mRNA expression.CONCLU-SION:Combination of octreotide with Dachaihu decoction effectively attenuates SAP by inhibiting NF-κB signaling pathway and ICAM-1 and IL-1 expression.
