Expression of calprotectin in rats with renal ischemia-reperfusion injury
10.3969/j.issn.1000-4718.2016.07.027
- VernacularTitle:钙卫蛋白在肾脏缺血再灌注损伤大鼠中的表达及其作用
- Author:
Pingzhen CHEN
;
Ye ZHU
;
Huitao ZHANG
;
Xiaochang XU
;
Jing ZHENG
;
Ning JIA
;
Yujing LIN
;
Lingling LI
;
Hua ZHANG
- Publication Type:Journal Article
- Keywords:
Kidney;
Ischemia-reperfusion injury;
Calprotectin;
Toll-like receptor 4
- From:
Chinese Journal of Pathophysiology
2016;32(7):1317-1322
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To investigate the expression of calprotectin ( CALP) in the rats with renal ischemia-reperfu-sion injury ( IRI) .METHODS:Male Sprague-Dawley rats were randomly divided into sham operation and IRI group ( n=25 in each group) .Blood samples and the kidneys were obtained at 6 h, 12 h, 24 h, 48 h and 72 h after reperfusion.The pathological changes of the kidneys were observed.The serum concentrations of blood urea nitrogen ( BUN) and serum cre-atinine (SCr) were measured.The serum levels of CALP, tumor necrosis factor-α(TNF-α) and interleukin-6 (IL-6) were detected by ELISA, and the expression of CALP, Toll-like receptor 4 ( TLR4) and NF-κB p65 in the renal tissues were de-termined by the methods of immunohistochemistry and Western blot.RESULTS: Different serial ischemia changes were observed in the renal tissues, mainly in the renal tubular epithelial cells and the mesenchyma, with the infiltration of in-flammatory cells.The serum levels of BUN, SCr, CALP, TNF-αand IL-6 in IRI group were markedly increased as com-pared with sham group (P<0.05).The protein expression of CALP, TLR4 and NF-κB p65 in the renal tubular epithelial cells in IRI group was greatly enhanced in comparison with that in sham group ( P<0.05) .CONCLUSION:The serum concentrations of CALP, TNF-αand IL-6, and the protein expression levels of CALP, TLR4 and NF-κB p65 in the renal tissue are significantly increased in the rats with IRI, suggesting that calprotectin plays an important role in the inflamma-tion in rats with IRI.
