Study on phenotype and function of intestinal gamma delta T lymphocytes in mice with post-infectious irritable bowel syndrome
10.3969/j.issn.1000-484X.2016.07.005
- VernacularTitle:PI-IBS小鼠肠黏膜γδT细胞表型和功能变化的实验研究
- Author:
Xuchun ZHOU
;
Xiaoning SUN
;
Bo YANG
;
Baili HUANG
;
Taozhi DENG
;
Zhoutao HE
;
Xiangyang HAN
;
Sun DEMING
;
Cheng LAN
- Publication Type:Journal Article
- Keywords:
Post-infectious irritable bowel syndrome;
γδT cells;
Inflammation;
Phenotype;
Function
- From:
Chinese Journal of Immunology
2016;32(7):957-961
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the phenotype and function of the intestinal γδT lymphocytes in post-infectious irritable bowel syndrome mouse model. Methods:The mouse model for post-infectious irritable bowel syndrome was established by the infection with trichinella spiralis. The intestinal inflammation,abdominal withdrawal reflex( AWR) and colon transportation test were observed. 2 and 8 weeks later,the animals were sacrificed and the lymphocytes in the intestinal lymph nodes and spleen were collected,from which the γδT lymphocytes were isolated and purified by monoclonal antibody-immuno-microbeads method. The functions of the purified γδT lymphocytes were evaluated,including proliferation by 3 HTdR;CD69,CD62L molecule staining by flow cytometry. Furthermore,the con-centration of cytokine IL-17 and IFN-γ in the supernatant of the cultured γδT lymphocytes were detected by ELISA. Results: At 2nd weeks after infection,significant intestinal inflammation was observed,with increasingγδT lymphocytes,proliferating and activating with increasing production of IL-17. At 8th weeks after infection, the intestinal inflammation disappeared, whereas the number of γδT lymphocytes remained increasing,also with proliferating and activating with increasing production of IL-17. Meanwhile,the mice show higher AWR score and Bristol score. Conclusion: γδT lymphocytes could participate in the pathogenesis of PI-IBS via their proliferation,activation and production of IL-17.
