Matrix metalloproteinases-11 collabrotive matrix metalloproteinases-14 promote development of breast carcinoma
10.3969/j.issn.1000-484X.2016.07.014
- VernacularTitle:基质金属蛋白酶-11协同14促进乳腺癌发展
- Author:
Hongyan HUANG
;
Jinxiang TAN
;
Guosheng REN
- Publication Type:Journal Article
- Keywords:
Matrix metalloproteinase-11 and 14;
Breast carcinoma;
Migration;
Invasion
- From:
Chinese Journal of Immunology
2016;32(7):996-999
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression pattern of MMP-11 and MMP-14 in breast carcinoma, and the effect of MMP-11 on breast carcinoma cell migration and invasion. Methods:MMP-11 and MMP-14 expression were examined in 161 invasive breast carcinoma tissue samples and 10 normal breast tissue samples. siRNA was used to knockout MMP-11 in breast carcinoma cell line MB-231 and Transwells were used to evaluate changes in migration ability and invasion ability. Results:Both MMP-11 and MMP-14 were highly expressed in breast carcinoma samples,122 and 149 samples out of 161,respectively. The expression of both proteins were correlated with lymph node metastasis and TNM staging. After knockout of MMP-11,the expression of both proteins decreased in MB-231 cell line and experiments show that the cell′s migration and invasion abilities were significantly weakened. Conclusion:MMP-11 and MMP-14 could promote invasion and metastasis of breast carcinoma. Knockout of MMP-11 results in the downregulated expression of MMP-14,and the inhibition of breast carcinoma cell′s migration and invasion. They could be potential prognostic markers and treatment targets for of breast carcinoma.
