Primary culture of rabbit fibroblast-like synoviocytes using suspended explant culture method

VernacularTitle:悬浮组织块法分离培养兔成纤维样滑膜细胞
Author: Qin LIU ; Liping WANG ; Fang CHEN ; Lifeng CHEN ; Yi ZHANG
Publication Type:Journal Article
Keywords: Suspended explant culture method; Rabbit fibroblast-like synoviocytes; Isolation; Culture
From: Chinese Journal of Immunology 2016;32(7):1009-1012
CountryChina
Language:Chinese
Abstract: Objective:To isolate rabbit fibroblast-like synoviocytes(FLSs) by suspended explant culture method. Methods:The healthy rabbit joint synovial layers were obtained. The cells were cultured by suspended explant culture method and compared with the explants adherent culture method. The growth status and morphology were observed. The growth curve of the 3rd passage cells was measured by CCK-8 assay,and the expression of vimentin was tested by immunocytochemistry. Results: The FLS obtained by the two methods exhibited a spindle-shaped appearance and could rapidly expand. The cell growth curve was typical of S type, and the cells highly expressed vimentin. Conclusion:Primary culture of rabbit fibroblast-like synoviocytes by suspended explant culture method were successfully established. The method was simple and highly efficient. It provided a new method for the isolation of FLS in vitro.