Expression of progesterone receptor a protein in prokaryotic and preparation of monoclonal antibodies to PR-A
10.3969/j.issn.1000-484X.2016.07.018
- VernacularTitle:孕激素受体A型原核表达及单克隆抗体制备
- Author:
Xiaolei ZHAO
;
Jinyu ZHAI
;
Lingling LIU
;
Yongbo LIANG
;
Yinyin NIU
;
Sanhua LI
;
Hua QI
- Publication Type:Journal Article
- Keywords:
Progesterone receptor;
Prokaryotic expression;
Monoclonal antibody
- From:
Chinese Journal of Immunology
2016;32(7):1013-1016
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare for mAb of progesterone receptor. It would provide support for the immunohistochemistry behind. Methods:Target gene connected together with a carrier by seamless cloning method. The target protein that expression by inducing was collected. And with cell fusion method , the monoclonal antibodies were preparation. Then the mAb were detected by IHC. Results: The mAb ( clone 7C7 ) was detected and it found positive for the breast, uterine fibroid tissue, showed negative in colorectal cancer tissue, smooth muscle tissue, the goal of the claim were achieve. Conclusion: Finally, we found the method that prepare for mAb was far beyond our imagination. The result of IHC on different samples about mAb(7C7)obtained compliance with an-ticipation. Study on the difference between the PR-A and PR-B had significance.
