Study on immune function and mechanism of T lymphocyte proliferation in patients with acute leukemia MSC
10.3969/j.issn.1000-484X.2016.07.028
- VernacularTitle:急性白血病患者MSC的免疫原性及抑制异体T淋巴细胞增殖功能和机制研究
- Author:
Ke LIU
;
Jingxia DU
;
Linhai RUAN
- Publication Type:Journal Article
- Keywords:
Acute leukemia;
Bone marrow mesenchymal stem cells;
Allogeneic;
T lymphocyte;
Proliferation;
Inhibition
- From:
Chinese Journal of Immunology
2016;32(7):1056-1059
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the inhibitory effect of bone marrow mesenchymal stem cells (MSC) on the proliferation of allogeneic T lymphocytes in patients with acute leukemia and its mechanism. Methods: 30 patients with acute myeloid leukemia ( AML) ,30 patients with acute lymphoblastic leukemia ( ALL) ,and 30 healthy subjects ( healthy group) were selected. MSC cells were isolated and cultured in three groups. The expression level of MSC cells in three groups were determined by MSC assay. The inhibitory effect of MSC on the proliferation of T cells was detected by Transwell assay. Results: AML group supernatant TGF-β1,HGF levels were significantly lower than the ALL group and the healthy group (P<0. 05),significantly higher than the levels of IL-11 ALL group of AML group,healthy group ( P<0. 05 ); three groups MSC supernatant IL-6 levels difference was not statistically significant ( P>0. 05). AML,ALL,MSC cells secrete cytokines healthy group of T lymphocyte proliferation still had a significant inhibitory effect, inhibition of differences in the case of contact and not in direct contact co-culture of T lymphocyte proliferation was not statistically sig-nificant (P> 0. 05); after joining the MSC,T cell proliferation in the three groups was significantly inhibited upon addition of anti-TGF-β1 antibody,anti-HGF antibody after T cell proliferation effective than a simple reversal join MSC (P<0. 05). Conclusion:MSC for acute leukemia patients inhibit allogeneic T lymphocyte proliferation,functioning principle may be related to the secretion of cyto-kines.
