Effect of Geraniin on Expression of Wnt3a in BMSC from Osteoporotic Rats
- VernacularTitle:老鹳草素对骨质疏松大鼠BMSCWnt3a表达的影响
- Author:
Li LI
;
Yue ZHANG
;
Renhua YANG
;
Mengdi WANG
;
Ting FU
;
Yuqiong YANG
;
Peng CHEN
;
Zhiqiang SHEN
- Publication Type:Journal Article
- Keywords:
Geraniin;
Osteoporosis;
Bone marrow stromal cells;
Wnt3a
- From:
Journal of Kunming Medical University
2016;37(6):9-12
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of geraniin on expression of Wnt3a protein and mRNA in bone marrow stromal cell (BMSC) from osteoporotic rats. Methods The model of osteoporosis (OP) was duplicated by ovariectomy in rats. BMSCs were isolated and cultured. BMSCs from shamed rats were routinly cultured and taken as normal control, and BMSCs from OP rats were divided into model group, 1μmol/L simvastatin positive group, and geraniin group (0.01, 0.1, 1, 10, 100 μmol/L), respectively. The methods of realtime-PCR and western blot were used to assay the protein and mRNA expression of wnt3a, respectively.Results As compared with normal control group, the protein and mRNA expression of wnt3a in model group were significantly suppressed;Compared with model group, 1 μmol/L simvastatin, and 0.1, 1, 10 and 100 μmol/L geraniin significantly increased the expression of wnt3a protein and mRNA. Conclusion It is suggested that geraniin activates wnt/β-catenin pathway though increasing the expression of signaling protein wnt 3a in BMSCs from OP rats. It would be beneficial to osteogenic differentiation of BMSCs and osteogenesis.
