Effects of sinapic acid on proliferation and apoptosis of rat vascular smooth muscle cells induced by high glucose
10.3969/j.issn.1000-4718.2016.07.004
- VernacularTitle:芥子酸对高糖诱导下大鼠血管平滑肌细胞增殖和凋亡的影响
- Author:
Xing PEI
;
Yong HAN
;
Zhanhua ZHANG
;
Na LI
;
Yao SHI
;
Yuanyuan ZHANG
;
Yigang FAN
;
Hongyan TIAN
- Publication Type:Journal Article
- Keywords:
Sinapic acid;
High glucose;
Vascular smooth muscle cells;
Cell proliferation;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2016;32(7):1174-1179
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To investigate the effects of sinapic acid ( SA) on the proliferation and apoptosis of rat vas-cular smooth muscle cells (VSMCs) induced by high glucose (HG).METHODS:Cultured A7r5 cells were randomly di-vided and treated as indicated.The cell viability was determined by MTT assay.DNA synthesis was measured by BrdU as-say.Cell cycle progression and cell apoptotic rate were determined by flow cytometry analysis.The levels of reactive oxygen species (ROS) were detected by ELISA.The protein levels of cyclin D1, P21, P27, phosphorylated protein kinase C (p-PKC), p-P38 andβ-actin were evaluated by Western blot.RESULTS:Compared with control group, the viability of A7r5 cells was significantly enhanced, the DNA synthesis was increased, the cell cycle progression was promoted, the levels of ROS were elevated, the cell apoptotic rate was reduced, the protein expression of P21 and P27 was decreased, and the pro-tein levels of cyclin D1, p-PKC and p-P38 were increased in HG group (all P<0.05).These effects were reversed by SA (0.1, 1 and 10 μmol/L) treatment in a dose-dependent manner (all P<0.05).Both P38 inhibitor SB203580 and PKC inhibitor chelerythrine significantly inhibit HG-induced PKC/P38 activation and cell viability ( P <0.05).CONCLU-SION:SA inhibits HG-induced VSMCs proliferation and promotes cell apoptosis via reducing PKC/P38 activation.
