Effect of MG132 on Aβgeneration in SH-SY5Y cells
10.3969/j.issn.1000-4718.2016.07.007
- VernacularTitle:MG132对SH－SY5Y细胞Aβ水平的影响
- Author:
Hao WANG
;
Lili SUN
;
Yang YU
;
Yanru YANG
;
Jian MA
;
Wei CHEN
;
Jiguo ZHANG
;
Shucun QIN
- Publication Type:Journal Article
- Keywords:
Alzheimer’s disease;
Ubiquitin-proteasome system;
MG132;
Beta-amyloid protein;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2016;32(7):1195-1199
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To observe the influences of different concentrations of MG132 on apoptosis and beta-amyloid protein ( Aβ) generation in SH-SY5Y cells, and to explore the underlying mechanism.METHODS:SHSY-5Y cells were incubated with MG132 for 24 h.The final concentrations of MG132 were 2.5, 5 and 10μmol/L.The cell viability was de-termined by MTT assay.The cell apoptosis was assessed by flow cytometry.The levels of Aβwere measured by ELISA. The relative protein levels were detected by Western blot.RESULTS:In the SH-SY5Y cells, MG132 reduced the cell via-bility, induced the cell apoptosis, increased the level of Aβ, and increased the expression of the related proteins for Aβgeneration in a concentration-dependent manner.CONCLUSION: MG132 induces apoptosis and increases the levels of Aβ1-42 and Aβ1-40 by regulating the proteins related to Aβgeneration in the SH-SY5Y cells.
