Effect of miR-200b on intestinal epithelial tight junction via MLCK/P-MLC signaling pathway

VernacularTitle:miR-200b调控肌球蛋白轻链激酶/磷酸化肌球蛋白轻链信号通路对肠上皮紧密连接的影响
Author: Yujie SHEN ; Cong ZHANG ; Yingwei CHEN
Publication Type:Journal Article
Keywords: miR-200 b; intestinal epithelial tight junction; myosin light chain kinase; phosphorylated myosin light chain; cell
From: Journal of Clinical Pediatrics 2016;34(7):540-543
CountryChina
Language:Chinese
Abstract: Objective To explore the impact and mechanism of miR-200 b on intestinal epithelial tight junction. Methods The negative-lentivirus and human-miR-200 b-lentivirus were employed to infect the Caco-2 cell thus establishing two stable cell lines which were then stimulated by 10 ng/mL human tumor necrosis factor-α(TNF-α) to establish the model of the intestinal epithelial injury. Those Caco-2 cells were divided into NC, NC+TNF-α, 200b, and 200b+TNF-αgroups.The tight junction permeability was detected by transepithelial electrical resistance (TEER) and Fluorescein isothiocyanate-labeled dextran (FITC-dextran). The protein alterations myosin light chain kinase (MLCK)/phosphorylated myosin light chain (P-MLC) pathways were measured by Western blot analysis. Results Compared to NC group, NC+TNF-αgroup had lower TEER, higher FITC-dextran, and up-regulated expressions of MLCK and P-MLC proteins (P