Exploration of the regulation site of HIF-1α in Notch signaling pathway in the hippocampus of rats with status ;epilepticus
10.3969/j.issn.1000-3606.2016.07.016
- VernacularTitle:癫痫持续状态大鼠海马内Notch通路对HIF-1α调控位点的研究
- Author:
Yafei ZHUANG
;
Lin ZHANG
;
Baoqiang YUAN
;
Yuanyuan DAI
;
Rui LI
- Publication Type:Journal Article
- Keywords:
status epilepticus;
Notch signaling pathway;
hippocampus;
rat
- From:
Journal of Clinical Pediatrics
2016;34(7):544-548
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of hypoxia inducible factor-1 (HIF-1 ) and Notch signaling pathway downstream gene HES 1 in the hippocampus of pubertal rats with status epilepsy (SE), and to explore the regulation site of HIF-1αin Notch signaling pathway. Methods One hundred and seventy-six 21-day-old SD rats were randomly divided into control group (NS group), pentetrazole (PTZ)-induced SE group (PTZ group), and Notch signaling pathway speciifc inhibitor (DAPT) intervention group (DAPT group). In PTZ group PTZ was intraperitoneally injected to build SE model and in NS group normal saline was injected as control. The intraperitoneal injection of diazepam was used to terminate SE seizures. After successful modeling, the bilateral hippocampuses were isolated after the rats were sacriifced at 0.5, 1, 2, 4 and 8 h, respectively, and RT-PCR was performed to detect the mRNA expression of HES 1 and HIF-1α. The Western Blot was performed to detect protein expression in hippocampuses which were collected at 2 , 4 , 8 , 12 , and 24 h after successful modeling. DAPT group received intraperitoneal injection of DAPT 30 min before the start of molding, then the hippocampuses were isolated at 2 and 8 h after successful modeling. RT-PCR was performed to detect the mRNA expression of HES 1 and HIF-1αat 2 h, and Western blot was performed to detect protein expression at 8 h. Results At each time point after SE, the expression of mRNA of HES 1 and HIF-1αand the expression of protein were higher than the same time point of NS group (P
