The protective effect of Clostridium butyricum on intestinal injure in newborn rats with necrotizing enterocolitis
10.3969/j.issn.1000-3606.2016.07.017
- VernacularTitle:酪酸梭菌对坏死性小肠结肠炎新生鼠肠损伤的保护作用
- Author:
Yance HE
;
Xiangying HE
;
Yongkun HUANG
;
Qunwen XIAO
;
Qi LI
;
Jie ZENG
;
Caiying ZHANG
- Publication Type:Journal Article
- Keywords:
Clostridium butyricum;
necrotizing enterocolitis;
vascular endothelia growth factor;
preliferating cell nuclear antigen;
claudin-2 protein
- From:
Journal of Clinical Pediatrics
2016;34(7):549-554
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of Clostridium butyricum on the expression of vascular endothelial cell growth factor (VEGF) and its receptor 2 (VEGFR-2), proliferating cell nuclear antigen (PCNA), and tight junction protein claudin-2 in intestinal tissue in newborn rat with necrotizing enterocolitis (NEC). Methods Forty-eight-hour-old Sprague-Dewley (SD) rats were randomly divided into model group, control group, low-dose group, mid-dose group, and high-dose group, 12 rats each. Rats in each group were fed with milk substitute. The NEC model were created by hypoxia and cold stimulation for 3 consecutive days in model group, low-dose group, mid-dose group, and high-dose group. Meanwhile, low-dose group, mid-dose group, and high-dose group were intervened by being fed with Clostridium butyricum 0.2, 0.4, and 0.8 g/(kg·d), respectively. All rats in each group were sacriifced on day 4 and the intestines tissue was obtained. The pathological changes had been observed. The expression of VEGF, PCNA, and claudin-2 were detected by immunohistochemistry. The expression of VEGFR-2 was detected by RT-PCR. Results The intestines pathological scores was signiifcantly different among ifve groups (P?0 . 05 ). Conclusion The expression of VEGF, VEGF-2 , and claudin-2 were higher in rats with NEC, while the expression of PCNA was lower. Supplementation of Clostridium butyricum may protect newborn rats by its act on these factors.
