Effects of lentivirus-mediated siRNA interference of USP39 on proliferation and migration of mice vascular smooth muscle cell
10.3969/j.issn.1005-1678.2016.06.09
- VernacularTitle:慢病毒介导siRNA靶向下调USP39表达对小鼠血管平滑肌细胞增殖及迁移的影响
- Author:
Shuai HE
;
Li YIN
;
Wei ZHONG
;
Zhibing QIU
- Publication Type:Journal Article
- Keywords:
lentivirus;
USP39;
VSMC;
proliferation;
migration
- From:
Chinese Journal of Biochemical Pharmaceutics
2016;36(6):38-40
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of lentivirus-mediated siRNA interference of USP39 on proliferation and migration of mice vascular smooth muscle cell in vitro.Methods Five siRNAs of siControl, siRNAUSP39-70, siRNAUSP39-71, siRNAUSP39-72 and siRNAUSP39-73 were designed and sythezied,mice VSMCs were infected with the lentivirus for delivering siRNAUSP39-73, and the stably transfected cells were selected by puromycin.The interference efficiency of siRNAUSP39-73 was assessed with Western blot.The effect of USP39 interference on the proliferation of VSMCs was determined by cells counting and MTT assay.Transwell assay was used to detect the migration of VSMCs.Results Recombinant lentiviral vector siRNAUSP39-73 was successfully transfected into mice VSMCs.Comparing with siControl group and Normal group, USP39 protein level of siRNAUSP39-73 VSMCs were decreased(P<0.05), and the proliferation and migration ability were all inhibited(P<0.05).Conclusion Targeted down-regulation of USP39 expression can inhibit the proliferation and migration of mice VSMCs in vitro.
