Simultaneous determination of xylitol and L-xylulose in fermentation broth with high performance liquid chromatography
10.3969/j.issn.1005-1678.2016.06.58
- VernacularTitle:高效液相色谱法同时测定发酵液中木糖醇和L－木酮糖的含量
- Author:
Chiyu GE
;
Jie JIANG
;
Xu WENG
;
Junli ZHANG
- Publication Type:Journal Article
- Keywords:
high performance liquid chromatographic(HPLC);
simultaneous determination;
xylitol;
L-xylulose;
fermentation broth
- From:
Chinese Journal of Biochemical Pharmaceutics
2016;36(6):191-193
- CountryChina
- Language:Chinese
-
Abstract:
Objective A high performance liquid chromatographic (HPLC) method was established for the simultaneous determination of xylitol and L-xylulose in fermentation broth.Methods The chromatographic conditions were as follows:C18 column (250 mm ×4.6 mm) with the temperature 35℃, acetonitrile-water (85∶15,v/v)as mobile phase with the flow rate of 0.8 mL/min.Xylitol was detected by refractive index (RI) detector at 33℃and L-xylulose was determined by ultraviolet ( UV) detector at 210 nm at room temperature.Results This method showed good linearity over the range from 0.50~30.00 g/L with a correlation coefficient of 0.9995 for xylitol and 0.30~30.00 g/L with a correlation coefficient of 0.9986 for L-xylulose. Moreover, the limit of quantification (LOQ) for xylitol and L-xylulose were 0.58 and 0.40,respectively.The limit of determination (LOD) for xylitol and L-xylulose were 0.18 and 0.15,respectively.The relative standard deviations (RSDs) of intraday and interday for xylitol were less than 0.64%and 0.80%,respectively.The intraday and interday RSDs for L-xylulose were less than 0.31%and 0.59%.The recoveries of xylitol and L-xylulose in fermentation broth were between 99.00%-101.00%.Conclusion There was no interference from other constitutes in the fermentation broth by this method.The methods were suitable for the simultaneous determination of the substrate xylitol and the product L-xylulose in fermentation process.
