Determination of glucosamine hydrochloride and related substances in glucosamine and indometacin enteric-coated capsules by HPLC-ELSD
10.3969/j.issn.1005-1678.2016.06.59
- VernacularTitle:HPLC－ELSD法测定氨糖美辛肠溶胶囊中盐酸氨基葡萄糖及相关物质
- Author:
Tailiang WANG
;
Ziqin WANG
- Publication Type:Journal Article
- Keywords:
glucosamine hydrochloride;
HPLC-ELSD;
content determination;
related substances
- From:
Chinese Journal of Biochemical Pharmaceutics
2016;36(6):194-196
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method for determination of glucosamine hydrochloride and its related substances in glucosamine and indometacin enteric-coated capsules.Methods Separation was carried out on a Inertsil C8 Column (250 mm ×4.6 mm,5μm) with a mobile phase composed of acetonitrile-water(70∶30) at a flow rate of 1.0 mL/min and the column temperature was 25℃.Results The linear ranges of calibration curve was 25 -312.5 μg/mL(r=0.9991, n=6).The average recovery was 98.0%, and the relative standard deviation was 1.3%( n =5 ) .The glucosamine hydrochloride was stable with acid destruction, but unstable with alkali, heat and strong light destruction.Conclusion The method is simple, accurate and reproducible for determination of glucosamine hydrochloride and related substances.
