Effects of Acetyl-l-carnitine on Autophagy, Apoptosis, and Locomotor Function after Acute Spinal Cord Injury in Rats
10.3969/j.issn.1006-9771.2016.07.003
- VernacularTitle:乙酰左旋肉碱对急性脊髓损伤大鼠细胞自噬、凋亡及运动功能的影响
- Author:
Qingfeng MENG
;
Mingchao ZHANG
;
Wei LU
;
Yunlong BI
;
Zhongkai FAN
- Publication Type:Journal Article
- Keywords:
acute spinal cord injury;
acetyl-l-carnitine;
autophagy;
apoptosis;
rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2016;22(7):754-758
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of acetyl-l-carnitine (ALC) on autophagy, apoptosis and motor function after acute spinal cord injury (ASCI) in rats. Methods Thirty-six adult female Sprague-Dawley rats were randomly divided into sham operation group (Sham group, n=12), simple spinal cord injury group (SCI group, n=12), ALC treatment group (ALC group, n=12). Spinal cord injury model at the level of T10 segment was established using Allen's method. They were assessed with Basso-Beattle-Bresnahan (BBB) scale three days after injury. Then the rats were sacrificed, and the expression of microtubule associated protein 1 light chain 3 (LC3)-II in spinal cord was detect-ed with Western blotting and immunofluorescent labeling, and the number of apoptotic cells were assessed with TUNEL staining. Results The expression of LC3-II and the number of apoptotic cells increased in SCI group compared with those in Sham group (P<0.01), while the BBB score decreased (P<0.001). The expression of LC3-II increased and the number of apoptotic cells decreased in ALC group compared with those in SCI group (P<0.001), while the BBB score increased (P<0.01). Conclusion ALC may promote autophagy, and inhibit apopto-sis to improve the locomotor function after ASCI.
